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B113
Growth medium · 8 cell lines grown in it
At a glance
- Base
- Gamborg B5 salts
- pH
- 5.8
- Gelling agent
- Gelrite 5 g
- Autoclave
- 121 °C, 15 to 20 min
- Vitamins
- After autoclaving
Lab recipe
For 1 liter, in the order it is made
Last changed on or before , while the collection was at UMass Amherst. Not yet reviewed at Tufts.
1 Dissolve the components
B5 Salts & Organics 3.1 g Phytotechlab G7682,4-Dichlorophenoxyacetic acid (2,4-D) 0.1 mL of a 10 mg/mL stock Alfa Aesar A12467Kinetin 0.1 mL of a 1 mg/mL stock Alfa Aesar 177254Glutamine 1.46 g Alfa Aesar A14201Sucrose 30 g Fisher S5*122 Make up to 1 L with water and adjust the pH to 5.8
3 Add the gelling agent
Gelrite 5 g Plantmedia.com 30634003-44 Autoclave at 121 °C for 15 to 20 minutes
5 Add the vitamin solution, then pour
Gamborg B5 Vitamin Mix, 112 mg/mL (1,000X) 1 mL Plantmedia.com 30630027-1
Cell lines grown in B113
8 cell lines
| Culture ID | Species | Status | Growth |
|---|---|---|---|
| AL-09736-T4 | Abronia latifolia | Growth 2 of 5 | |
| CC-04683-T4-L2 | Ceanothus 'Concha' | Growth 2 of 5 | |
| CF-08150-F2 | Cryptantha flava | Not recorded | |
| MV-10980 | Malva verticillata | Growth 2 of 5 | |
| MP-12539 | Mirbelia pungens | Growth 3 of 5 | |
| NB-12890 | Nertera balfouriana | Growth 1 of 5 | |
| OT-11491-T4 | Ouratea sp. | Growth 2 of 5 | |
| SC-11806 | Serjania cuspidata | Growth 2 of 5 |
Cite this medium
https://pccl.tufts.edu/medium/B113