B39

Growth medium · 3 cell lines grown in it

Lab recipe

For 1 liter, in the order it is made

Last changed on or before 3 October 2024, while the collection was at UMass Amherst. Not yet reviewed at Tufts.

1 Dissolve the components
B5 Salts & Organics 3.1 g Phytotechlab G768
2,4-Dichlorophenoxyacetic acid (2,4-D) 0.5 mL of a 10 mg/mL stock Alfa Aesar A12467
Sucrose 20 g Fisher S5*12
2 Make up to 1 L with water and adjust the pH to 5.8
3 Add the gelling agent
Gelrite 5 g Plantmedia.com 30634003-4
4 Autoclave at 121 °C for 15 to 20 minutes
5 Add the vitamin solution, then pour
Gamborg B5 Vitamin Mix, 112 mg/mL (1,000X) 1 mL Plantmedia.com 30630027-1

B5 Salts & Organics	3.1 g	Phytotechlab `G768`
2,4-Dichlorophenoxyacetic acid (2,4-D)	0.5 mL of a 10 mg/mL stock	Alfa Aesar `A12467`
Sucrose	20 g	Fisher `S5*12`

Gelrite	5 g	Plantmedia.com `30634003-4`

Gamborg B5 Vitamin Mix, 112 mg/mL (1,000X)	1 mL	Plantmedia.com `30630027-1`

3 cell lines

Culture ID	Species	Status	Growth
AS-04252-R0 C1	Acanthus spinosus	Cryopreserved	Growth 2 of 5
AR-02190-L2	Ajuga reptans	Cryopreserved	Growth 2 of 5
HF2244	Hypericum forrestii	Cryopreserved	Growth 2 of 5

https://pccl.tufts.edu/medium/B39