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B5
Growth medium · 8 cell lines grown in it
At a glance
- Base
- Gamborg B5 salts
- pH
- 5.8
- Gelling agent
- Gelrite 7 g
- Autoclave
- 121 °C, 15 to 20 min
- Vitamins
- After autoclaving
Lab recipe
For 1 liter, in the order it is made
Last changed on or before , while the collection was at UMass Amherst. Not yet reviewed at Tufts.
1 Dissolve the components
B5 Salts & Organics 3.1 g Phytotechlab G7682,4-Dichlorophenoxyacetic acid (2,4-D) 0.1 mL of a 10 mg/mL stock Alfa Aesar A12467Kinetin 0.1 mL of a 1 mg/mL stock Alfa Aesar 177254Sucrose 20 g Fisher S5*122 Make up to 1 L with water and adjust the pH to 5.8
3 Add the gelling agent
Gelrite 7 g Plantmedia.com 30634003-44 Autoclave at 121 °C for 15 to 20 minutes
5 Add the vitamin solution, then pour
Gamborg B5 Vitamin Mix, 112 mg/mL (1,000X) 1 mL Plantmedia.com 30630027-1
Cell lines grown in B5
8 cell lines
| Culture ID | Species | Status | Growth |
|---|---|---|---|
| AM-00332-R1 | Abelmoschus moschatus | Growth 1 of 5 | |
| AM-00007-L1 | Arnica montana | Growth 1 of 5 | |
| CM-00808-L2 C1 | Cotoneaster monopyrenus | Growth 2 of 5 | |
| CP-00723-G12 | Cotoneaster pruinosus | Growth 1 of 5 | |
| DL-02088-R0 C2 | Digitalis lutea | Growth 1 of 5 | |
| FR2083-E1 | Ficus religiosa | Growth 1 of 5 | |
| PD-05726-F3 | Pleiospilos compactus subsp. canus | Not recorded | |
| TU-00029 T4 S1 | Turnera ulmifolia | Growth 1 of 5 |
Cite this medium
https://pccl.tufts.edu/medium/B5