B53

Last changed on or before 3 October 2024, while the collection was at UMass Amherst. Not yet reviewed at Tufts.

1. 1 Dissolve the components

   B5 Salts & Organics	3.1 g	Phytotechlab G768
   2,4-Dichlorophenoxyacetic acid (2,4-D)	0.2 mL of a 10 mg/mL stock	Alfa Aesar A12467
   Kinetin	0.2 mL of a 1 mg/mL stock	Alfa Aesar 177254
   α-Naphthaleneacetic Acid (NAA) Solution	0.5 mL of a 1 mg/mL stock	Phytotechlab N605
   Indole-3-Acetic Acid (IAA)	0.5 mL of a 1 mg/mL stock	Phytotechlab I885
   Casein Hydrolysate	2 g	Spectrum C3626
   Sucrose	20 g	Fisher S5*12

2. 2 Make up to 1 L with water and adjust the pH to 5.8

3. 3 Add the gelling agent

   Agar	10 g	Fisher BP-1423-500

4. 4 Autoclave at 121 °C for 15 to 20 minutes

5. 5 Add the vitamin solution, then pour

   Gamborg B5 Vitamin Mix, 112 mg/mL (1,000X)	1 mL	Plantmedia.com 30630027-1