B6

Last changed on or before 3 October 2024, while the collection was at UMass Amherst. Not yet reviewed at Tufts.

1. 1 Dissolve the components

   B5 Salts & Organics	3.1 g	Phytotechlab G768
   6-Benzylaminopurine (6-BA)	1 mL of a 1 mg/mL stock	Phytotechlab B130
   α-Naphthaleneacetic Acid (NAA) Solution	1 mL of a 1 mg/mL stock	Phytotechlab N605
   Sucrose	20 g	Fisher S5*12

2. 2 Make up to 1 L with water and adjust the pH to 5.8

3. 3 Add the gelling agent

   Gelrite	5 g	Plantmedia.com 30634003-4

4. 4 Autoclave at 121 °C for 15 to 20 minutes

5. 5 Add the vitamin solution, then pour

   Gamborg B5 Vitamin Mix, 112 mg/mL (1,000X)	1 mL	Plantmedia.com 30630027-1

No cell line in the catalog lists B6 as its medium.