B64

Growth medium

Lab recipe

For 1 liter, in the order it is made

Last changed on or before 3 October 2024, while the collection was at UMass Amherst. Not yet reviewed at Tufts.

1 Dissolve the components
B5 Salts & Organics 3.1 g Phytotechlab G768
Kinetin 0.01 mL of a 1 mg/mL stock Alfa Aesar 177254
α-Naphthaleneacetic Acid (NAA) Solution 8 mL of a 1 mg/mL stock Phytotechlab N605
Sucrose 20 g Fisher S5*12
2 Make up to 1 L with water and adjust the pH to 5.5
3 Add the gelling agent
Gelrite 5 g Plantmedia.com 30634003-4
4 Autoclave at 121 °C for 15 to 20 minutes
5 Add the vitamin solution, then pour
Gamborg B5 Vitamin Mix, 112 mg/mL (1,000X) 1 mL Plantmedia.com 30630027-1

B5 Salts & Organics	3.1 g	Phytotechlab `G768`
Kinetin	0.01 mL of a 1 mg/mL stock	Alfa Aesar `177254`
α-Naphthaleneacetic Acid (NAA) Solution	8 mL of a 1 mg/mL stock	Phytotechlab `N605`
Sucrose	20 g	Fisher `S5*12`

Gelrite	5 g	Plantmedia.com `30634003-4`

Gamborg B5 Vitamin Mix, 112 mg/mL (1,000X)	1 mL	Plantmedia.com `30630027-1`

No cell line in the catalog lists B64 as its medium.

https://pccl.tufts.edu/medium/B64