B89

Growth medium · 11 cell lines grown in it

Lab recipe

For 1 liter, in the order it is made

Last changed on or before 3 October 2024, while the collection was at UMass Amherst. Not yet reviewed at Tufts.

1 Dissolve the components
B5 Salts & Organics 3.1 g Phytotechlab G768
2,4-Dichlorophenoxyacetic acid (2,4-D) 0.1 mL of a 10 mg/mL stock Alfa Aesar A12467
Kinetin 0.1 mL of a 1 mg/mL stock Alfa Aesar 177254
Sucrose 30 g Fisher S5*12
2 Make up to 1 L with water and adjust the pH to 5.8
3 Add the gelling agent
Gelrite 5 g Plantmedia.com 30634003-4
4 Autoclave at 121 °C for 15 to 20 minutes
5 Add the vitamin solution, then pour
Gamborg B5 Vitamin Mix, 112 mg/mL (1,000X) 1 mL Plantmedia.com 30630027-1

B5 Salts & Organics	3.1 g	Phytotechlab `G768`
2,4-Dichlorophenoxyacetic acid (2,4-D)	0.1 mL of a 10 mg/mL stock	Alfa Aesar `A12467`
Kinetin	0.1 mL of a 1 mg/mL stock	Alfa Aesar `177254`
Sucrose	30 g	Fisher `S5*12`

Gelrite	5 g	Plantmedia.com `30634003-4`

Gamborg B5 Vitamin Mix, 112 mg/mL (1,000X)	1 mL	Plantmedia.com `30630027-1`

11 cell lines

Culture ID	Species	Status	Growth
AR-00161-T4-R1	Alcea rosea	Cryopreserved	Growth 2 of 5
CC-01256	Chrysocoma coma-aurea	Cryopreserved	Growth 1 of 5
CO-05412	Cirsium odontolepis	Cryopreserved	Growth 2 of 5
CP-06336-T4-L1	Cotoneaster pannosus	Cryopreserved	Growth 1 of 5
EW1627-L1	Euphorbia wulfenii	Cryopreserved	Growth 1 of 5
GB-06546	Globularia vulgaris	Cryopreserved	Growth 2 of 5
LA-02467-S1	Lysimachia atropurpurea	Cryopreserved	Growth 1 of 5
MA-02921	Matthiola arborescens	Cryopreserved	Growth 1 of 5
MS-00459	Mitragyna speciosa	Cryopreserved	Growth 1 of 5
OB-05510	Onopordum bracteatum	Cryopreserved	Growth 1 of 5
SD-05879	Scoparia dulcis	Cryopreserved	Growth 1 of 5

https://pccl.tufts.edu/medium/B89