M128

Growth medium · 8 cell lines grown in it

Lab recipe

For 1 liter, in the order it is made

Last changed on or before 3 October 2024, while the collection was at UMass Amherst. Not yet reviewed at Tufts.

1 Dissolve the components
MS Salts & Organics 4.33 g Caisson MSP01-50LT
2,4-Dichlorophenoxyacetic acid (2,4-D) 0.5 mL of a 10 mg/mL stock Alfa Aesar A12467
Kinetin 0.1 mL of a 1 mg/mL stock Alfa Aesar 177254
Sucrose 30 g Fisher S5*12
2 Make up to 1 L with water and adjust the pH to 5.8
3 Add the gelling agent
Gelrite 5 g Plantmedia.com 30634003-4
4 Autoclave at 121 °C for 15 to 20 minutes
5 Add the vitamin solution, then pour
Murashige and Skoog (MS) Vitamin Powder Mix, 103.1mg/mL (1,000X) 1 mL Plantmedia.com 30630198-1

MS Salts & Organics	4.33 g	Caisson `MSP01-50LT`
2,4-Dichlorophenoxyacetic acid (2,4-D)	0.5 mL of a 10 mg/mL stock	Alfa Aesar `A12467`
Kinetin	0.1 mL of a 1 mg/mL stock	Alfa Aesar `177254`
Sucrose	30 g	Fisher `S5*12`

Gelrite	5 g	Plantmedia.com `30634003-4`

Murashige and Skoog (MS) Vitamin Powder Mix, 103.1mg/mL (1,000X)	1 mL	Plantmedia.com `30630198-1`

8 cell lines

Culture ID	Species	Status	Growth
CC-14163	Capsicum chinense	Cryopreserved	Growth 2 of 5
CP-13047	Cymbopogon pospischilii	Cryopreserved	Not recorded
FV-13042	Festuca valesiaca	Cryopreserved	Growth 3 of 5
LR-12925	Lolium remotum	Cryopreserved	Growth 1 of 5
PP-12958	Phleum pratense	Cryopreserved	Growth 1 of 5
TV-13736	Triticum aestivum	Cryopreserved	Growth 2 of 5
TT-13040	Triticum timopheevii	Cryopreserved	Growth 1 of 5
VS-14400-C1	Vigna unguiculata subsp. sesquipedalis	Cryopreserved	Growth 4 of 5

https://pccl.tufts.edu/medium/M128