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M42
Growth medium · 7 cell lines grown in it
At a glance
- Base
- Murashige & Skoog (MS) salts
- pH
- 6
- Gelling agent
- Agar 14 g
- Autoclave
- 121 °C, 15 to 20 min
- Vitamins
- After autoclaving
Lab recipe
For 1 liter, in the order it is made
Last changed on or before , while the collection was at UMass Amherst. Not yet reviewed at Tufts.
1 Dissolve the components
MS Salts & Organics 4.33 g Caisson MSP01-50LT2,4-Dichlorophenoxyacetic acid (2,4-D) 0.02 mL of a 10 mg/mL stock Alfa Aesar A12467α-Naphthaleneacetic Acid (NAA) Solution 0.18 mL of a 1 mg/mL stock Phytotechlab N605Sucrose 30 g Fisher S5*122 Make up to 1 L with water and adjust the pH to 6
3 Add the gelling agent
Agar 14 g Fisher BP-1423-5004 Autoclave at 121 °C for 15 to 20 minutes
5 Add the vitamin solution, then pour
Murashige and Skoog (MS) Vitamin Powder Mix, 103.1mg/mL (1,000X) 1 mL Plantmedia.com 30630198-1
Cell lines grown in M42
7 cell lines
| Culture ID | Species | Status | Growth |
|---|---|---|---|
| EP-00775-R0 | Echium pininana | Growth 2 of 5 | |
| EC-14389 | Eriodictyon crassifolium | Growth 2 of 5 | |
| EC-14345 | Erythrina corallodendron | Growth 1 of 5 | |
| GS-00022-L2 | Gaertnera sp. | Growth 2 of 5 | |
| HA-07630-R1 C1 | Helichrysum aureonitens | Growth 1 of 5 | |
| HA-00994-R1 | Herniaria alpina | Growth 2 of 5 | |
| PM-12783-F1 | Polypogon monspeliensis | Not recorded |
Cite this medium
https://pccl.tufts.edu/medium/M42