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M91
Growth medium · 4 cell lines grown in it
At a glance
- Base
- Murashige & Skoog (MS) salts
- pH
- 5.8
- Gelling agent
- Gelrite 5 g
- Autoclave
- 121 °C, 15 to 20 min
- Vitamins
- After autoclaving
Lab recipe
For 1 liter, in the order it is made
Last changed on or before , while the collection was at UMass Amherst. Not yet reviewed at Tufts.
1 Dissolve the components
MS Salts & Organics 4.33 g Caisson MSP01-50LT2,4-Dichlorophenoxyacetic acid (2,4-D) 0.2 mL of a 10 mg/mL stock Alfa Aesar A12467Kinetin 0.2 mL of a 1 mg/mL stock Alfa Aesar 177254Coconut Water 100 mL Vita Coco Sucrose 30 g Fisher S5*122 Make up to 1 L with water and adjust the pH to 5.8
3 Add the gelling agent
Gelrite 5 g Plantmedia.com 30634003-44 Autoclave at 121 °C for 15 to 20 minutes
5 Add the vitamin solution, then pour
Murashige and Skoog (MS) Vitamin Powder Mix, 103.1mg/mL (1,000X) 1 mL Plantmedia.com 30630198-1
Cell lines grown in M91
4 cell lines
| Culture ID | Species | Status | Growth |
|---|---|---|---|
| EG-12499-G2 | Enneapogon gracilis | Growth 2 of 5 | |
| GF-12671 | Gaudinia fragilis | Growth 2 of 5 | |
| PA-12859 | Phalaris arundinacea | Growth 1 of 5 | |
| PT-12891 | Poa trivialis | Not recorded |
Cite this medium
https://pccl.tufts.edu/medium/M91