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POM

Growth medium

Base
Phytotech Orchid Replate Medium
pH
5.8
Gelling agent
Gelrite 5 g
Autoclave
121 °C, 15 to 20 min
Vitamins
After autoclaving

Lab recipe

For 1 liter, in the order it is made

Last changed on or before , while the collection was at UMass Amherst. Not yet reviewed at Tufts.

  1. 1 Dissolve the components

    Phytotech Orchid Replate Medium35.81 gPhytotechlab P781

  2. 2 Make up to 1 L with water and adjust the pH to 5.8

  3. 3 Add the gelling agent

    Gelrite5 gPlantmedia.com 30634003-4

  4. 4 Autoclave at 121 °C for 15 to 20 minutes

  5. 5 Add the vitamin solution, then pour

    Gamborg B5 Vitamin Mix, 112 mg/mL (1,000X)1 mLPlantmedia.com 30630027-1

Cell lines grown in POM

No cell line in the catalog lists POM as its medium.

Cite this medium

https://pccl.tufts.edu/medium/POM